Plasmid transformation of Azospirillum brasilense
نویسندگان
چکیده
منابع مشابه
Intermediary carbon metabolism of Azospirillum brasilense.
Azospirillum brasilense Sp 7 grew rapidly in AZO medium containing reduced nitrogen and succinate as an energy source, with a doubling time of 43 min. No growth was measured with glucose as the sole carbon source. In contrast, Azospirillum lipoferum Sp 59b could grow in media containing either succinate or glucose with a doubling time of 69 min and 223 min, respectively. Warburg-Barcroft respir...
متن کاملCellular Responses during Morphological Transformation in Azospirillum brasilense and Its flcA Knockout Mutant
FlcA is a response regulator controlling flocculation and the morphological transformation of Azospirillum cells from vegetative to cyst-like forms. To understand the cellular responses of Azospirillum to conditions that cause morphological transformation, proteins differentially expressed under flocculation conditions in A. brasilense Sp7 and its flcA knockout mutant were investigated. Compari...
متن کاملRepressor mutant forms of the Azospirillum brasilense NtrC protein.
The Azospirillum brasilense mutant strains FP8 and FP9, after treatment with nitrosoguanidine, showed a null Nif phenotype and were unable to use nitrate as their sole nitrogen source. Sequencing of the ntrC genes revealed single nucleotide mutations in the NtrC nucleotide-binding site. The phenotypes of these strains are discussed in relation to their genotypes.
متن کاملExtended phenotype of an mreB-like mutant in Azospirillum brasilense.
Tn5 mutagenesis was used to generate an Azospirillum brasilense SPF94 mutant. Genetic analysis of this mutant revealed that a homologue of the mreB gene, which controls cell shape in Bacillus subtilis and Escherichia coli, was inactivated. The cell-surface properties of the mutant were different from those of the parental strain. The mutant colonies were highly fluorescent when grown on plates ...
متن کاملTemperature inhibition of siderophore production in Azospirillum brasilense.
The effect of growth at 42 degrees C on the different components of the siderophore-mediated iron transport that are induced by iron limitation in Azospirillum brasilense was examined. Biosynthesis of the siderophore spirilobactin was strongly inhibited (20-fold) by growth at 42 degrees C, whereas the transport of iron by the ferric-spirilobactin transport system and the induction of the iron-r...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1986
ISSN: 0378-1097
DOI: 10.1016/0378-1097(86)90155-2